BL2 Laboratory Manual Suggested Table of Contents

Each Principal Investigator (PI) in a BL2 laboratory must develop and maintain a Laboratory Specific Biosafety Manual for their lab.  This Biosafety Manual should: 1) contain safety information relevant to the lab’s specific hazards and research materials, 2) serve as a training tool for personnel and include documentation of such training, 3) be readily available to all research personnel in the lab and 4) be modified as needed to contain current laboratory SOPs and practices.

At a minimum, the laboratory Biosafety manual should include:

  • Lab contact information (PI name, phone number, including after hours number)
    • Relevant sections of the Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5thEdition, (e.g. Sections I, II, and III pertaining to standard  BL1 and BL2 practices, Appendix H, pertaining to work with Human cells and tissues  if applicable).

    • A list of Agents used and Agent Summary Statement(s) or MSDS for  each agent used in the lab;
    • Project specific SOPs;
    • Laboratory specific Biosafety policies, including:
    • PPE required for working in the lab
    • Infectious Waste Disposal procedures for:
        1. Liquids
        2. Sharps
        3. Solids
        4. Research Animals (if applicable)
      • Biohazardous Spill Clean-Up Procedures
    • Copy of current IBC approval letter
    • copies of recent laboratory inspection report(s).
    • Documentation of lab/agent specific training (date and topics covered) provided by PI (or designee) to all personnel working in the lab, signed by PI and personnel.